Metabolomics
Services

Expert Metabolite &
Small Molecule Services

The metabolome is an entire set of low-molecular weight molecules (typically <1,500 Da), commonly known as metabolites, within a biological system. Metabolomics, the study of the metabolome, aims to identify and quantify metabolites including their substrates, intermediates and end products in biosynthesis, metabolism, degradation and salvage; which concentrations are influenced by both genetic and environmental factors.  MRM Proteomics Inc. offers metabolomics services in two ways — targeted metabolomics and untargeted metabolomics.

Targeted Metabolomics


MRM Proteomics offers 20 different services for targeted metabolomics. Our services allow for reliable quantitation of ~1000 metabolites & small molecules from key biochemical pathways or specific compound classes.

MRM Proteomics Inc. offers a variety of targeted  metabolomics and other small moclecule analysis services in a compound class  or pathway-specific manner for your metabolomics needs. Targeted metabolite services encompass the use of an array of custom-developed UPLC-MRM/MS methods, in combination of isotope dilution and/or chemical derivatizaiton techniques , to provide high-specificity, high-selectivity, and high-reproducibility detection and quantitation of hundreds of known metabolites in diverse biological samples such as body fluids, tissues, cells, culture media and plants. To make our services compatible for a variety of research demands, we have fully optimized sample preparation for several complex biofluids and tissue types in differing model organisms.  Precise and accurate quantitation of specific and targeted metabolites in human samples offers a promising route to disease diagnosis, therapeutic management and prognostic prediction in clinical applications.

  • Description

    • Quantification of metabolites in folate cycle, methione cycle, choline metabolism and transsulfuration pathway by LC-MS

    • 2x10(6) cells/50 mg tissues


  • Description

    • Quantification of glucose, sugar phosphates, CoAs, enzyme co-factors, TCA cycle carboxylic acids, phosphocarboxylic acids and 9 major nucleotides involved in Central Carbon Metabolism (CCM) by UPLC-MRM/MS

    • 2x10(6) cells/50 mg tissues


  • Description

    • Quantitation of amino acids, amines and selected metabolic intermediates

    • 50 µL plasma/serum, 2x10(6) cells, 2x10(5) neurons or 50 mg tissues


  • Description

    • Quantification of sphingolipid biosynthesis intermediates, sphinganines, ceramides, sphingomyelins and selected gangliosides by UPLC-MRM/MS

    • 50 µL plasma/serum, 2x10(6) cells, 2x10(5) neurons or 50 mg tissues


  • Description

    • Detection and quantitation of all known isoprenyl phosphate intermediates, isoprenoids (ubiquinones, dolichols, vitain K2 and squalene, etc.), cholesterol and other sterols by UPLC-MRM/MS and UPLC-FTMSdisease progression

    • 50 mg tissues, 1x10(7) cells


  • Description

    • Simultaneous quantification of medium- to long-chain fatty acids and acyl-carnitines by UPLC-MRM/MS

    • 50 µL plasma/serum, 2x10(6) cells, 2x10(5) neurons or 50 mg tissues


  • Description

    • Identification & quantification of nucleotides, nucleobases and nucleosides by UPLC-MRM/MS

    • 200 µL urine, 50 mg tissues, 1x10(7) cells


  • Description

    • Quantification of in vivo vitamins and metabolites of host-gut co-metabolism by UPLC-MRM/MS

    • 100 µL plasma/serum or 100 mg tissues

Pathway Specific Services

Class Specific Services

  • Description

    • Quantification of 51 unconjugated, taurine- and glycine-conjugated bile acids in human and rodent plasma/serum, bile, tissues, cells, cecal content, brain, neurons, urine and feces by UPLC-(-)MRM/MS

    • 25 µL plasma/serum, 1 µL bile, 200 µL urine, 2x10(6) cells or 20 mg tissues


  • Description

    • Quantitation of 51 compounds in bile acids analysis I and 16 bile acid sulfates and glucuronides in plasma/serum and urine in human and rodent plasma/serum, bile, tissues, cells, cecal content, brain, neurons, urine and feces by UPLC-(-)MRM/MS

    • 25 µL plasma/serum, 1 µL bile, 200 µL urine, 2x10(6) cells or 20 mg tissues


  • Description

    • Quantitation of 2 precursors of bile acid synthesis (7 ?-OH chol-4-esten-3-one and 7?,12?-diOH chol-4-esten-3-one) in human and rodent plasma/serum, bile, tissues, cells, cecal content, brain, neurons, urine and feces by UPLC-(+)MRM/MS.

    • 50 µL plasma/serum, 2x10(6) cells or 50 mg tissues


  • Description

    • Quantification of ribose, xylose, ribulose, arabinose, rhamnose, xylulose, fucose, glucose, mannose, galactose, fructose, maltose, lactose, cellobiose by UPLC-MRM/MS

    • 50 µL plasma/serum, 2x10(6) cells or 50 mg tissues


  • Description

    • UPLC-(FT)MS/MS identification and UPLC-FTMS quantitation of cardiolipin compounds in cell, tissues, CSF and blood samples

    • 100 µL plasma/serum, 2x10(6) cells or 50 mg tissues


  • Description

    • Quantification of small molecule aldehydes (MDA, 4-HHE, 4-HNE, and C2 to C9 aldehydes) by chemical derivatization UPLC-MRM/MS

    • 100 µL plasma/serum and 0.5 mL urine


  • Description

    • Quantification of about 30 steroid hormones by UPLC-MRM/MS

    • 100 µL plasma/serum, 100 mg tissues


  • Description

    • Quantitation of 10 2nd nucleotide messengers

    • 2x10(6) cells or 50 mg tissues


  • Description

    • Identification and quantification of 21 plant phytohormones by UPLC-MRM-MS

    • 100-300 mg, depending on tissue. Please inquire.


  • Description

    • Quantitation of 10 C2 to C6 short-chain fatty acids

    • 20 mg tissues


  • Description

    • Quantitation of short- to very long-chain (C2 to C26) saturated/unsaturated, even-/odd-carbon number, hydroxyl and di-carboxylic fatty acids

    • 20 mg tissues


  • Description

    • Quantitation of free and C2 to C18 acyl-CoAs

    • 50 mg tissues, 1x10(7) cells

Untargeted Metabolomics


MRM Proteomics Inc. offers a variety of untargeted metabolomics services that are ideal for comparative studies — we will assess an unlimited number of samples.

Untargeted metabolomics is a discovery-based research approach focusing on detection and relative quantitation of thousands of known and unknown metabolites in the metabolome of a given biological system.  This technology is most advantageously used in comparative studies, to identify the changes in the metabolome among two or more experimental sample groups. MRM Proteomics Inc. uses UPLC coupled to high-resolution and high-mass accuracy MS instrumentation along with well established metabolome database searching  and quality control procedures to confidently determine the metabolite concentration changes. We also provide similar services for analysis of different lipid classes. As the field of metabolomics continues to mature, untargeted metabolomics is an important aspect of biomarker discovery in biological and clinical studies.

Untargeted Service Types

  • Description

    • Untargeted profiling and relative quantification of known and unknown metabolites by UPLC-MS with 4 LC-MS methods with (+) and (-) ESI detection

    • 100 µL plasma/serum, 2x10(6) cells or 50 mg tissues


  • Description

    • Untargeted profiling and relative quantification of lipids in plasma/serum, cells, tissues, plants and bacteria by UPLC-FTMS with (+) and (-) ESI detection

    • 100 µL plasma/serum, 2x10(6) cells or 50 mg tissues


  • Description

    • Various lipids (phospholipids, glycerol lipids, fatty acids, acyl carnitines, etc.) in mammalian and plant tissues by MALDI-FTMS with (+) and (-) ion detection

    • Whole organs or tissues


  • Description

    • Major sugar phosphates, nucleotides, nucleotide-sugar conjugates

    • Whole organs or tissues